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Silencing Fas-associated phosphatase 1 expression enhances efficiency of chemotherapy for colon carcinoma with oxaliplatin

Silencing Fas-associated phosphatase 1 expression enhances efficiency of chemotherapy for colon carcinoma with oxaliplatin

作     者:Navada Eagleton 

作者机构:Department of Veterinary Physiology and PharmacologyTexas A&M UniversityCollege Station 

出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))

年 卷 期:2010年第16卷第1期

页      面:112-118页

核心收录:

学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基  金:Supported by Research grants from the Science and Technology Foundation of Guangdong Province,No.2006B36002010,2008B030301092,2009B030801005 the Foundation of Health Department of Guangdong Province,No.A2005226 the foundation of Guangzhou Science and Technology Bureau,No. 2009Y-C011-1 the Natural Science Foundation of Guangdong Province,No.7001592 the National Natural Science Foundation of China,No.30973505 

主  题:Colon carcinoma Fas-associated phosphatase 1 RNA interference Oxaliplatin Chemotherapy 

摘      要:AIM:To investigate whether silencing Fas-associated phosphatase 1(FAP-1)expression enhances the efficiency of chemotherapy for colon carcinoma with ***:Expression of FAP-1 in mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR)and flow *** interfering RNA(siRNA)was designed according to the FAP-1 mRNA *** proliferation was evaluated by methyl thiazolyl tetrazolium(MTT)*** V-and propidine iodine(PI)were assayed by flow cytometry for the detection of apoptosis. RESULTS:The expression of FAP-1 was increased in SW480 cells after chemotherapy with oxaliplatin. Transfection of FAP-1 siRNA into SW480 cells silenced the expression of FAP-1 and consequently abolished the inhibitory function of Fas/FasL-mediated apoptosis pathway,thus increasing the efficacy of chemotherapy for colon carcinoma with oxaliplatin. CONCLUSION:RNA interference combined with conventional chemotherapy is more effective against colon cancer.

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