Highly oxygenated dihydrostilbenoids and flavones from the aerial part of Glycyrrhiza uralensis and their antimicrobial activities

作     者：Xinyi Wei Junfei Zhou Liping Zeng Mingming Xu Chuntao Che Jin’ao Duan Hui Yan Hongkai Bi Ming Zhao 

作者机构：Jiangsu Collaborative Innovation Centre of Chinese Medicinal Resources IndustrializationNational and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative MedicineSchool of PharmacyNanjing University of Chinese MedicineNanjingChina Department of Pharmaceutical SciencesCollege of Pharmacythe University of Illinois ChicagoChicagoILUSA Department of Pathogen BiologyJiangsu Key Laboratory of Pathogen BiologyNanjing Medical UniversityNanjingChina 

出 版 物：《Science of Traditional Chinese Medicine》 (中医药科学（英文）)

年 卷 期：2024年第2卷第4期

页      面：291-302页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：supported financially by the Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization(ZDXM-2020-17) Jiangsu Medical Specialist Programs of China,the Jiangsu Specially Appointed Professor,and Jiangsu Province“Innovative and Entrepreneurial Team”Program 

主　　题：Phenolic compounds Electronic circular dichroism(ECD) Bactericidal activity Helicobacter pylori 

摘      要：Background:Multidrug-resistant bacteria(MDRB)represent a significant global challenge due to their high mortality rates,substantial economic burden,and rapid *** triple or quadruple therapies have demonstrated limited efficacy as a result of increasing drug ***,it is urgent to develop novel anti-MDRB drugs with high efficiency and low ***:To isolate and identify the dihydrostilbenoids and flavones from the aerial part of Glycyrrhiza uralensis(Fabaceae)and to screen their antimicrobial *** and methods:The aerial part of *** was extracted with 75%aqueous *** crude extract was repeatedly isolated by macroporous resin,silica gel,Sephadex LH-20,C_(18)-MPLC,and MCI-MPLC,which were then purified by semipreparative RPHPLC to obtain monomer *** structures of the isolates were assigned by a combination of optical rotations,UV spectra,nuclear magnetic resonance,and high-resolution electrospray ionization mass spectrometry,and the absolute configurations of compounds 2,3,and 7 were further confirmed by electronic circular dichroism ***,we investigated their antimicrobial activities by the broth microdilution ***:Seventeen previously undescribed phenolic compounds(1–17)and 26 known analogs(18–43),including dihydrostilbenoids,flavones,and dihydroflavones,were identified from the aerial part of *** vitro,antimicrobial bioassays demonstrated that compound 31 displayed the strongest inhibitory effect against 4 drug-resistant Helicobacter pylori strains(MIC=2–4μg/mL),comparable to metronidazole(MIC=1–32μg/mL).Additionally,compounds 10,13,and 15 demonstrated bactericidal activity against Staphylococcus aureus(MIC=4μg/mL),while compounds 15 and 22 exhibited inactivation effects against Mycobacterium smegmatis,Enterococcus faecium,and ***(MIC=4–8μg/mL).Conclusions:These monomeric compounds with antimicrobial activities were isolated from the aerial parts