Observation on Plant Leaf Transection by Gelatin Blotting

作     者：TAN Dahai LI Fuheng WANG Xiaocen HUANG Fushan 

作者机构：College of Life Sciences Northeast Agricultural University Harbin 150030 China Resources and Environmental Sciences College Northeast Agricultural University Harbin 150030 China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2011年第18卷第3期

页      面：7-11页

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：Supported by Committee of Nature Science Foundation of Heilongjiang Province (C2005-32) Post-doctoral Science Committee of China (LRB04-217) Scientific Research Start Committee of Northeast Agricultural University 

主　　题：gelatin solution blotting leaf transaction observation 

摘      要：Blotting was used to observe cell structures of leaf epidermis cells, and the key method of leaf transaction observation was paraffin section. The concentration, suitable solidification time, melting temperature of gelatin solution and the stain for the gelatin blotting were studied in this research. The results showed that the gelatin blotting could be used to study leaf transaction, it was benefit to make operation easily, and save time, money and so on