Localized,non-viral delivery of nucleic acids:Opportunities,challenges and current strategies

作     者：Oliver Germershaus Kira Nultsch 

作者机构：Institute for Pharma TechnologySchool of Life SciencesUniversity of Applied Sciences and Arts Northwestern SwitzerlandGründenstrasse 40CH 4132MuttenzSwitzerland 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2015年第10卷第3期

页      面：159-175页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 07[理学] 

主　　题：Gene delivery Topical application Tissue engineering Controlled release SiRNA DNA 

摘      要：Localized delivery of drugs is an emerging field both with regards to drug delivery during disease as well as in tissue *** significant achievements made in the last decades,the efficient delivery of proteins and peptides remains challenging,especially in cases requiring long-term release of proteins after *** localized delivery of nucleic acids(NA)represents an interesting alternative due to higher physicochemical stability of NA,increased efficiency by harnessing cells as bioreactors for the production of required proteins and improved versatility with regards to expression of specific proteins through plasmid DNA or repression of gene products through ***,unlike most proteins and peptides,NA must be delivered to the cytoplasm or nucleus to be efficacious,resulting in significant delivery *** herein describe frequently used non-viral vectors for the delivery of NA including polyplexes,lipoplexes and lipopolyplexes and summarize recent developments in the field of nucleic acid delivery systems for local application based on hydrogels,solid scaffolds and physical delivery *** challenges associated with the different approaches are identified and options to address these challenges are discussed.