Simultaneous separation and determination of four main isoflavonoids in Astragali Radix by an isocratic LC/ESI-MS method

作     者：王玉林 梁逸曾 张洁 冯晓亮 葛承胜 黄兰芳 WANG Yu-ling;LIANG Yi-zeng;ZHANG Jie;FENG Xiao-liang;GE Cheng-sheng;HUANG Lan-fang

作者机构：Department of Chemical and Materials EngineeringQuzhou College College of Chemistry and Chemical EngineeringCentral South University 

出 版 物：《Journal of Central South University》 (中南大学学报（英文版）)

年 卷 期：2016年第23卷第2期

页      面：303-309页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 070302[理学-分析化学] 1008[医学-中药学(可授医学、理学学位)] 0810[工学-信息与通信工程] 1006[医学-中西医结合] 0806[工学-冶金工程] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project(21472110)supported by the National Natural Science Foundation of China Project(LY15B050008)supported by the Natural Science Foundation of Zhejiang Province,China Project(2013Y003)supported by Quzhou Technology Projects,China 

主　　题：liquid chromatography electrospray ionization (ESI) mass spectrometric detection (MS) isoflavonoids Astragali Radix 

摘      要：A simple, reliable and rapid isocratic liquid chromatography(LC)-mass spectrometric detection(MS) coupled with electrospray ionization(ESI) method for simultaneous separation and determination of calycosin-7-O-β-D-glucoside, ononin, calycosin and formonometin in Astragali Radix was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using water and acetonitrile(70:30, v/v) containing 0.2%(v/v) acetic acid as a mobile phase and a 2.0 mm×150 mm Hypersil-Keystone C18 column. Selective ion monitoring(SIM) mode and [M+H]+ ions at m/z 447, 431, 285 and 269 were used for quantitative analysis of four main active components above mentioned. The calibration curves were linear in the range of 0.4-175.0 μg/mL for calycosin-7-O-β-D-glucoside, 0.2-146.0 μg/m L for ononin, 0.4-210.0 μg/mL for calycosin and 0.5-217.0 μg/mL for formonetion, respectively. The limits of quantification(LOQ) and detection(LOD) were 0.4 μg/mL and 0.08 μg/m L for calycosin-7-O-β-D-glucoside, 0.2 μg/mL and 0.06 μg/m L for ononin, 0.4 μg/mL and 0.1 μg/mL for calycosin, 0.5 μg/m L and 0.1 μg/m L formonetion, respectively. The standard recoveries were in the range of 96.5%-104.7%. The developed method has successfully been used for the determination of four main flavonoids in Astragali Radix from various sources and can be used for identification, differentiation and quality evaluation of Astragali Radix.