RAPID DETECTION OF EXOGENOUS HUMAN CLOTTING FACTOR IX cDNA IN TRANSFERRED CELLS BY POLYMERASE CHAIN REACTION

作     者：周洁民 戴一凡 邱信芳 薛京伦 

作者机构：Institute of Genetics Fudan University Shanghai 200433 PRC 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1990年第35卷第23期

页      面：1995-1999页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Project supported by High Technique Research of the State 

主　　题：polymerase chain reaction human clotting factor Ⅸ cDNA exogenous cDNA detection. 

摘      要：Polymerase chain reaction (PCR) is a recently devoloped molecular biological technique, using specific oligonucleotide primers and DNA polymerase to amplify the specific fragment of DNA in vitro. This technique has been successfully introduced in gene diagnosis, mutation detection, polymorphism analysis and nucleotide sequencing. In the study of gene transfer and gene therapy, it is necessary to analyse DNA in the