Fluorescence lifetime imaging of molecular rotors to map microviscosity in cells

作     者：James A. Levitt Marina K. Kuimova Gokhan Yahioglu Pei-Hua Chung Klaus Suhling David Phillips 

作者机构：Department of Physics King's College London Department of Chemistry Imperial College London PhotoBiotics Ltd. 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2010年第8卷第10期

页      面：926-930页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：UK's Engineering and Physical Sciences Research Council (EPSRC) UK's Biotechnology and Biological Sciences Research Council (BBSRC) King's College London Graduate School 

主　　题：Fluorescence lifetime imaging of molecular rotors to map microviscosity in cells 

摘      要：Fluorescence liftime imaging （FLIM） of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incubating cells with these dyes, we find a punctate and continuous distribution of the dye in cells. The viscosity value obtained in what appears to be endocytotic vesicles in living cells is around 100 times higher than that of water and of cellular ***-resolved fluorescence anisotropy measurements also yield rotational correlation times consistent with large microviscosity values. In this way, we successfully develop a practical and versatile approach to map the microviscosity in cells based on imaging fluorescent molecular rotors.