Generation of Induced Pluripotent Stem Cells with High Efciency from Human Umbilical Cord Blood Mononuclear Cells

作     者：Juan Wang Qi Gu Jie Hao Donghui Bai Lei Liu Xiaoyang Zhao Zhonghua Liu Liu Wang Qi Zhou 

作者机构：State Key Laboratory of Reproductive BiologyInstitute of ZoologyChinese Academy of Sciences College of Life ScienceNortheast Agricultural University of China Graduate School of Chinese Academy of Sciences 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2013年第11卷第5期

页      面：304-311页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 0714[理学-统计学（可授理学、经济学学位）] 0703[理学-化学] 0701[理学-数学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 10[医学] 

基　　金：supported in part by grants from China National Basic Research Program of China(Grant No.2012CBA01301 to QZ,2013CB967102 to LL and 2012CB966500 to XZ) the ‘‘Strategic Priority Research Program’’ of the Chinese Academy of Sciences(Grant No.XDA01030101)to LW 

主　　题：Human UCBMCs Human iPSCs Dox-inducible lentiviralsystem 

摘      要：Human induced pluripotent stem cells （iPSCs） hold great promise for regenerative med- icine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononu- clear cells （UCBMCs） is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs （UCB-iPSCs） have characteristics that are identical to pluripotent human embryonic stem cells （hESCs）. This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.