Biological effects of low-dose-rate irradiation of pancreatic carcinoma cells in vitro using ^(125)I seeds
Biological effects of low-dose-rate irradiation of pancreatic carcinoma cells in vitro using ^(125)I seeds作者机构：Department of Radiology Ruijin Hospital Luwan Branch Shanghai Jiao Tong University School of Medicine Institution of Molecular Imaging Southeast University Nanjing 210009 China Department of Radiology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Soochow University School of Medicine Department of Radiobiology School of Radiological Medicine and Protection Soochow University Institution of Molecular Imaging Southeast University Department of Interventional Radiology Shanghai St. Luke’s Hospital
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2015年第21卷第8期
页 面：2336-2342页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by Natural Science Foundation of China,No.81271682(partly) grants from Science and Technology Commission of Shanghai Municipality,No.11JC1407400(partly) the project of Luwan District Science and Technology Commission of Shanghai,No.LKW1104(partly) the project of Medical Key Specialty of Shanghai Municipality,No.ZK2012A20(partly)
主 题：125I radioactive seeds Biological effects Pancreat
摘 要：AIM:To determine the mechanism of the radiationinduced biological effects of 125I seeds on pancreatic carcinoma cells in ***:SW1990 and PANC-1 pancreatic cancer cell lines were cultured in DMEM in a suitable ***’s model of iodine-125(125I)seed irradiation was *** vitro,exponential phase SW1990,and PANC-1cells were exposed to 0,2,4,6,and 8 Gy using 125I radioactive seeds,with an initial dose rate of 12.13c Gy/h.A clonogenic survival experiment was performed to observe the ability of the cells to maintain their clonogenic capacity and to form ***-cycle and apoptosis analyses were conducted to detect the apoptosis percentage in the SW1990 and PANC-1 *** synthesis was measured via a tritiated thymidine(3H-Td R)incorporation *** continuous low-dose-rate irradiation with 125I radioactive seeds,the survival fractions at 2 Gy(SF2),percentage apoptosis,and cell cycle phases of the SW1990 and PANC-1 pancreatic cancer cell lines were calculated and ***:The survival fractions of the PANC-1 andSW1990 cells irradiated with 125I seeds decreased exponentially as the dose *** significant difference in SF2 was observed between SW1990 and PANC-1 cells(0.766±0.063 vs 0.729±0.045,P0.05).The 125I seeds induced a higher percentage of apoptosis than that observed in the control in both the SW1990and PANC-1 *** rate of apoptosis increased with increasing radiation *** percentage of apoptosis was slightly higher in the SW1990 cells than in the PANC-1 ***-dependent G2/M cellcycle arrest was observed after 125I seed irradiation,with a peak value at 6 *** the dose increased,the percentage of G2/M cell cycle arrest increased in both cell lines,whereas the rate of DNA incorporation *** the 3H-Td R incorporation experiment,the dosimetry results of both the SW1990 and PANC-1cells decreased as the radiation dose increased,with a minimum at 6 *** were no significant differences in the do
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