Self-assemblies of plasmonic gold/layered double hydroxides with highly efficient antiviral effect against the hepatitis B virus

作     者：Gabriela Carja Elena Florentina Grosu Catalina Petrarean Norica Nichita 

作者机构：Department of Chemical Engineering Faculty of Chemical Engineering and Environmental Protection Technical University 'Gh. Asachi' of lasi Bd. Mangeron No. 71 lasi 700554 Romania Department of Viral Glycoproteins Institute of Biochemistry of the Romanian Academy Splaiul Independentei 296 Bucharest 060031 Romania 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2015年第8卷第11期

页      面：3512-3523页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0703[理学-化学] 0901[农学-作物学] 070301[理学-无机化学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supports from the Romanian National Authority for Scientific Research  CNCS-UEFISCDI and the Romanian Academy 

主　　题：plasmonic gold layered double hydroxides layered double hydroxide (LDH) antiviral effect HBV hepatitis cytotoxicity 

摘      要：Engineering complex nanocomposites that specifically target the hepatitis B virus （HBV） and overcome the limitations of current therapies such as limited efficacy and serious side effects is very challenging. Here, for the first time, the antiviral effect of engineered plasmonic gold and layered double hydroxide self-assemblies （AuNPs/LDHs） is demonstrated, using HBV as a model virus and hepatoma-derived HepG2.2.215 ceils for viral replication, assembly, and secretion of infectious virions and subviral particles. AuNPs/LDHs were obtained by a simple, cost-effective procedure in which small AuNPs （-3.5 nm） were directly obtained and organized on the surface of larger LDH nanoparticles （-150 nm） by exploiting the capability of MgLDH, ZnLDH, and MgFeLDH to manifest their ＂structural memory＂ in the aqueous solution of Au（O2CCH3）3. The self-assembly approach of AuNPs and LDHs was assessed by transmission electron microscopy （TEM）, X-ray photoelectron spectroscopy （XPS）, powder X-ray diffraction （PXRD）, and UV-Vis analysis （UV-Vis）. All AuNPs/LDHs tested reduced the amount of viral and subviral particles released from treated cells by up to 80% and exhibited good cytocompatibility. AuNPs/MgFeLDH showed the highest antiviral HBV response with more than 90% inhibition of HBV secretion for the whole concentration range. Preliminary studies on the mechanism of HBV inhibition reveals that in the presence of AuNPs/LDHs, HBV particles are sequestered within the treated cells. The antiviral and low cytotoxic plasmonic properties of these Au/LDH nanocomposites indicate that they hold significant potential to be tailored as novel efficient therapeutics for the treatment of hepatitis B.