Matrix-assisted laser desorption/ionization time of flight mass spectrometry in the genetic basis of systemic lupus erythematosus and the complicating kidney injury

作     者：ZHOU Xiao-shuang LI Jing WANG Li-hua WANG Chen WU Rui-ping LI Rong-shan 

作者机构：Shanxi Med Univ Hosp 2 Dept Nephrol Taiyuan 030001 Shanxi Peoples R China Shanxi Med Univ Hosp 2 Dept Pathol Taiyuan 030001 Shanxi Peoples R China Shanxi Med Univ Taiyuan 030001 Shanxi Peoples R China Shanxi Prov Peoples Hosp Dept Nephrol Taiyuan 030012 Shanxi Peoples R China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2013年第126卷第15期

页      面：2821-2825页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 070302[理学-分析化学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：systemic lupus erythematosus " genetic diseases " kidney injury " matrix-assisted laser desorption/ionization-mas sspectrometry 

摘      要：Background It is necessary to develop some innovative methods to reveal and discover the novel （SLE）-related protein molecules. In the present study, matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI- TOF MS） was employed to detect the differential expression of serum polypeptides in the patients with systemic lupus erythematosus （SLE） presenting with a family history or complicating with kidney injury so as to identify the proteins associated with the genetic factors and kidney injury in SLE. Methods The subjects recruited were divided into four groups, that is, a group of SLE patients with both family history and kidney injury, a group of SLE patients with only kidney injury but no family history, a group of SLE patients with neither family history nor kidney injury, and a control group consisting of healthy volunteers. By adopting MALDI-TOF MS analysis, the serum samples obtained from the three groups of SLE patients were examined and compared with those from the control group; the categorized peptide fingerprint profile was established via the biological data collected from the samples. Results The expression of protein with a mlz of 4207 Da increased significantly in SLE patients; the protein with a ml z of 2658 Da was expressed in all SLE patients; three proteins （with mlz of 1465, 5332, and 5900 Da respectively） were expressed in the SLE patients complicated with kidney injury and the protein with a mlz of 1943 Da was expressed in SLE patients with family history. Conclusion A number of differential proteins were successfully detected and identified through MALDI-TOF MS detection and these proteins may be associated with the genetic basis of SLE and the complicating kidney injury.