Non-phospholipid liposomes with high sterol content display a very limited permeability

作     者：CARBAJAL Gustavo CUI Zhong-Kai LAFLEUR Michel 

作者机构：Department of ChemistryCenter for Self-assembled Chemical Structures (CSACS)Université de MontréalC.P.6128Succ.Centre VilleMontréalQuébec H3C 3J7Canada 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2013年第56卷第1期

页      面：40-47页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：financially supported by the Natural Sciences and Engineering Research Council of Canada by the Fonds Québécois de la Recherche sur la Nature et les Technologies through its Strategic Clusterprogram 

主　　题：liposomes nanovectors permeability pH gradient ascorbic acid 

摘      要：We demonstrate that it is possible to form non-phospholipid fluid bilayers in aqueous milieu with a mixture of palmitic acid (PA),cholesterol (Chol),and cholesterol sulfate (Schol) in a molar proportion of 30/28/*** self-assemblies are shown to be bilayers in the liquid ordered *** are stable between pH 5 and *** this pH range,the protonation/deprotonation of PA carboxylic group is observed but this change does not appear to alter the stability of these bilayers,a behavior contrasting with that observed for binary mixtures of PA/Chol,and PA/*** multilamellar dispersions formed spontaneously from the PA/Chol/Schol mixture could be successfully extruded to form Large Unilamellar Vesicles (LUVs).These LUVs show interesting permeability properties,linked with their high sterol *** non-phospholipid liposomes can sustain a pH gradient (pH internal 8/pH external 6) 100 times longer than LUVs made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol,with a molar ratio of 60/***,the non-phospholipid LUVs are shown to protect ascorbic acid from an oxidizing environment (1 mM iron(III)).Once entrapped in liposomes,ascorbic acid displays a degradation rate similar to that obtained in the absence of iron(III).These results show the possibility to form novel nanocontainers from a mixture of a monoalkylated amphiphile and sterols,with a good pH stability and showing interesting permeability properties.