Study on the Interaction of Colloidal Gold with Taq DNA Polymerase

作     者：朱红平 米丽娟 陈仕谋 王文锋 姚思德 

作者机构：Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai201800China Graduate University of Chinese Academ of SciencesBeijing100049China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2007年第25卷第9期

页      面：1233-1237页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：Project supported by the National Natural Science Foundation of China (No. 10675158) 

主　　题：Taq DNA polymerase colloidal gold conjugate biocatalytic activity polymerase chain reaction 

摘      要：The interaction of colloidal gold with Taq DNA polymerase （Taq） was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold.