Investigation on binding between cations and amides using UV Raman spectroscopy

作     者：Yi-xuan Liu[a] Zhou-bing Wang[a] Jing-jing Wang[a] Kang-zhen Tian[a][b] Xin Meng[a] Gui-lin Mao[a][b] 刘艺璇;王周兵;王静静;田康振;孟鑫;毛桂林

作者机构：[a]School of Physics and Electronic Engineering Jiangsu Normal University Xuzhou 221116 China [a]School of Physics and Electronic Engineering Jiangsu Normal University Xuzhou 221116 China[b]Jiangsu Key Laboratory of Advanced Laser Materials and Devices Xuzhou 221116 China 

出 版 物：《Chinese Journal of Chemical Physics》 (中国化学物理学报)

年 卷 期：2024年第37卷第4期

页      面：539-544页

核心收录：

学科分类：07[理学] 070203[理学-原子与分子物理] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(No.62005108,No.62205134) the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province(No.21KJB140008) the Graduate Research and Practice Innovation Program of Jiangsu Normal University(No.2021XKT1201,No.2021XKT1204) 

主　　题：UV Raman spectroscopy Butyramide Hofmeister 

摘      要：The interaction of proteins with salt ions plays an important role in life activities. We used butyramide as a model molecule to investigate the interaction of protein backbones with cations. The experiment was performed in an aqueous solution of metal chloride using UV Raman spectroscopy. It was found that well-hydrated metal cations (Ca2+, Mg2+) tend to bind to C=O in the amide bond, resulting in redistribution of the amide I band peaks. Specifically, the peak intensity ratio of 1655 cm-1 to 1610 cm-1 increases significantly with increasing concentrations. However, this phenomenon is not obviously observed in NaCl solution. Furthermore, we studied the effect of salt ions on the water structures. The addition of Ca2+ and Mg2+ is beneficial to the enhancement of the water signal at the 3400 cm−1 position, while the Na+ at the same concentration is not obvious. The results have shown that the interaction between cations and amides satisfies the following order: Ca2+Mg2+Na+, which conforms to the Hofmeister series.