Acute effect of aspartame-induced oxidative stress in Wistar albino rat brain

作     者：Iyaswamy Ashok Rathinasamy Sheeladevi Dapkupar Wankhar 

作者机构：Department of PhysiologyDr.ALM PG Institute of Basic Medical SciencesUniversity of MadrasSekkizhar Campus 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2015年第29卷第5期

页      面：390-396页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：financial assistance was provided by the University of Madras 

主　　题：aspartame blood methanol oxidative stress antioxidant free radical 

摘      要：The present study was carried out to investigate the acute effect of aspartame on oxidative stress in the Wistar albino rat brain. We sought to investigate whether acute administration of aspartame （75 mg/kg） could release methanol and induce oxidative stress in the rat brain 24 hours after administration. To mimic human methanol metabolism, methotrexate treated rats were used to study aspartame effects. Wistar strain male albino rats were administered with aspartame orally as a single dose and studied along with controls and methotrexate treated controls. Blood methanol and formate level were estimated after 24 hours and rats were sacrificed and free radical changes were observed in discrete regions by assessing the scavenging enzymes, reduce dglutathione （GSH）, lipid peroxidation and protein thiol levels. There was a significant increase in lipid peroxidation levels, superoxide dismutase activity （SOD）, glutathione peroxidase levels （GPx）, and catalase activity （CAT） with a significant decrease in GSH and protein thiol. Aspartame exposure resulted in detectable methanol even after 24 hours. Methanol and its metabolites may be responsible for the generation of oxidative stress in brain regions. The observed alteration in aspartame fed animals may be due to its metabolite methanol and elevated formate. The elevated free radicals due to methanol induced oxidative stress.