Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

作     者：Zhiyuan Li Zhanxiu Zhang Lili Zhao Hui Li Suxia Wang Yong Shen 

作者机构：Department of Joint OrthopedicsHebei Provincial Xingtai People’s Hospital Department of Spinal OrthopedicsThird Hospital of Hebei Medical University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第8期

页      面：806-814页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：nerve regeneration spinal cord injury bone marrow mesenchymal stem cells Nogo-66receptor RNA interference horseradish peroxidase BrdU gene silencing neural regeneration 

摘      要：We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was signiifcantly enhanced in the model group. Af-ter 8 weeks, the number of horseradish peroxidase-labeled nerve ifbers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and signiifcantly higher than in the model group. The newly formed nerve ifbers and myelinated ner ve ifbers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.