Variability in permeability and integrity of cell membrane and depletion of food reserves in neem(Azadirachta indica) seeds from trees of different age classes

作     者：evendra Kumar Dhruv Kumar Mishra 

作者机构：Seed Testing LaboratorySilviculture DivisionArid Forest Research Institute 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2014年第25卷第1期

页      面：147-153页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by Indian Council of Forestry Research and Education Dehradun 

主　　题：phospholipids α-Tocopherol Lipid peroxidation Azadirachta indica 

摘      要：We quantified cell membrane permeability （electrical conduc-tivity-EC, water soluble sugar-WSS, and amino acids-AA） and integrity （phospholipids,α-tocopherol and lipid peroxidation） along with food reserve deterioration （total proteins, total sugar, and total starch） of neem seeds collected from various mother tree age classes and stored for 65 days in airtight plastic containers at ambient room temperature （35±5°C）. Results show that the activities were higher in fresh seeds （EC 267.56-2950.01 μS/g, WSS 19.96-19.48 mg/g and AA 5.40-5.35 mg/g） and declined with increasing duration of storage period （EC 153.37-195.17 μS/g, WSS 3.13-4.17 mg/g and AA 4.29-4.49 mg/g after 35 days and EC 144.02-161.56 μS/g, WSS 2.06-2.40 mg/g and AA 3.98-4.27 mg/g after 65 days of storage）. Phospholipids andα-tocopherol were higher in fresh seed （0.073-0.093 OD at 710 nm and 0.080-0.105 OD, respectively） and declined as storage duration in-creased （0.033-0.042 OD at 710 nm and 00.0010-0.0020 OD, respec-tively）. Dead seeds showed reduced amounts of phospholipids and minimum activity ofα- tocopherol （antioxidants）. The level of MDA was lower in fresh seeds （0.0066-0.0087 OD at 600-535 nm） and increased as storage duration increased （0.0248-0.0268 OD after 65 days of stor-age）. The higher amount of MDA indicated that seeds died due to rancid-ity of the oil inside the seed. Neem seed cake was assessed for deteriora-tion of food reserves （total proteins, total sugar, and total starch）, concen-trations of which were higher in fresh seed and declined as storage dura-tion increased. Germination was higher in fresh seeds and after 65 days, no germination was received perhaps due to deterioration of biochemi-cals in seeds. Patterns of seed deterioration were similar across all seed lots.