Screening of Toxin Mutant of Dickeya zeae and Its Biological Characters

作     者：Jingyi ZHANG Yutao WANG Yanchang LI Qiongguang LIU 

作者机构：College of Natural Resource&EnvironmentSouth China Agricultural University 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2013年第14卷第10期

页      面：1397-1402页

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：Supported by the National Natural Science Foundation of China(31071658) 

主　　题：Dickeya zeae Toxin Virulence Extracellular enzyme Extracellular polysaccharide 

摘      要：[Objective]This study aimed to screen toxin mutant of Dickeya zeae(Erwinia chrysanthemi ***)and investigate its biological characters.[Method]We obtained a toxin mutant strain *** Ech7-3-42 by using acridine orange as a mutagenic agent and compared their biological characteristics and virulence between the toxin mutant and wild strain.[Result]There was no significant difference in pectin lyase,protease,cellulase and the production of extracellular polysaccharide and lipopolysaccharide,but significant difference in toxin biological activities and ***7-3-42 mutant did not produce toxin,as well as the loss of virulence on rice and HR on tobacco,but did not lose the ability to soft rot on *** strain Ech7-3-42 can infect rice root and then enriched in the root neck and stalk,but it could not cause rice foot *** zeae(wild and mutant strain)could be detected by PCR in the root neck and below the 1-2 cm long stem area,but could not be detected in the leaves.[Conclusion]We believed that toxin may be one of the important factors for *** virulence on rice.