Phosphorylation of histone H3 on Ser10 by auto-phosphorylated PAK1 is not essential for chromatin condensation and meiotic progression in porcine oocytes

作     者：Bingyuan Wang Wei Ma Xiaoling Xu Chao Wang Yubo Zhu Na An Lei An Zhonghong Wu Jianhui Tian 

作者机构：Ministry of Agriculture Key Laboratory of Animal Genetics Breeding and Reproduction National Engineering Laboratory for Animal Breeding College of Animal Science and Technology China Agricultural University Department of Histology and Embryology School of Basic Medical Sciences Capital Medical University Institute of Animal Husbandry and Veterinary Medicine Beijing Municipal Academy of Agriculture and Forestry Sciences 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2013年第4卷第2期

页      面：152-158页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by grants from the National High-Tech R&D Program (No. 2011AA100303) the National Key Technology R&D Program (No. 2011BAD19B01) the National Natural Science Foundation of China(No. 31271253) 

主　　题：Chromosome condensation H3^ser10 Meiotic progression PAK1^Thr423 Porcine oocyte 

摘      要：Background： The p21-activated kinase 1 （PAK1）is essential of microtubule assembly during oocyte meiotic maturation porcine oocytes. for mitosis and plays an important role in the regulatio in mice; however, little is known about its role in Result： Total p21-activated kinase 1 （PAK1） and phosphorylated PAK1 at Thr423 （PAK1^Thr423） were consistently expressed in porcine oocytes from the germinal vesicle （GV） to the second metaphase （MII） stages, but phosphorylation of histone H3 at Serr10 （H3^ser10） was only expressed after the GV stage. Immunofiuorescence analysis revealed that PAK1Thr423 and H3^ser10 colocalized on chromosomes after the GV stage. Blocking of endogenous PAK1^Thr423 by injecting a specific antibody decreased the phosphorylation level of H3^ser10; however, it had no impact on chromatin condensation, meiotic progression, cleavage rate of blastomeres or the rate of blastocyst formation. Conclusion： Phosphorylation of PAK1^Thr423 is a spontaneous activation process and the activated PAK1^Thr423 can promote the phosphorylation of H3^ser10; however, this pathway is not required for meiotic maturation of porcine oocytes or early embryonic development.