Protoplast Fusion of Cosmos bipinnatus and Cosmos sulphureus

作     者：Xiaoxia LIU Huoxu WANG 

作者机构：College of Life ScienceLiaoning Normal University Key Lab of Plant Biotechnology of Liaoning ProvinceLiaoning Normal University 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2013年第2卷第1期

页      面：24-26,41页

学科分类：090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 

主　　题：Cosmos bipinnatus Cosmos sulphureus Protoplast fusion 

摘      要：[ Objective] The aim was to study appropriate fusion conditions of protoplasts from 2 species Cosmos bipinnatus and Cosmos sulphureus. [ Method ] The protoplasts were separated from the blossom petals of Cosmos bipinnatus and Cosmos sulphureus availed with the function of pectinase and cellulase, and the fusion of two pretoplasts was performed with PEG. [ Results] A great deal of free pretoplasts were obtained from Cosmos bipinnatus by using 6% mannitol ＋ 1.3% cellu- lase ＋ 1.2% pectinase, and from Cosmos sulphureus by using 10% mannitol ＋ 1.3% cellulase ＋ 1.2% pectinase. Five hours after of enzymolysis treatment, large amount of dissociated protoplasts were observed in the solution. Pretoplast fusion was be obtained by using 30% PEG fusion liquid and 0. lmol/1 CaC12 solution （pH value = 9.5）. [ Conclusion] Our study established an optimal separation and fusion system of protoplasts from different coreopsis species, laying foundation for the breeding of novel coreopsis varieties.