Human Soluble TRAIL Protein Inducing Apoptosis in Osteosarcoma Cell

作     者：ZHU Shaobo YU Aixi ZHANG Zhongning WU Gang 

作者机构：Zhongnan Hospital Wuhan University Wuhan 430071Hubei China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2007年第12卷第6期

页      面：1148-1152页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the Natural Science Foundation of HubeiProvince (2003ABA163) Specialized Research Fund for the Doctoral Pro-gram of Higher Education (20060486049) 

主　　题：TRAIL (TNF-related apoptosis-inducing ligand) osteosarcoma cell line apoptosis 

摘      要：This study is to examine the effect of human recombinant soluble TRAIL （TNF-related apoptosis-inducing ligand） protein inducing apoptosis in MG-63 human osteosarcoma cells. The inhibitive rates of TRAIL to MG-63 cells were detected by MTT assay. The apoptosis induced by TRAIL in MG-63 human osteosarcoma cells was analyzed with FACS and TUNEL and the apoptotic bodies were observed by transmission electron microscope. MTT assay showed that the inhibitive rates of 500, 1 000, 2 000 and 4 000 ng/mL TRAIL for 24 h were 10.1%, 24.3%, 50.6% and 97.7% respectively. Flow cytometric analysis showed that after MG-63 cells were treated with 2 gg/mL TRAIL for 6 h, obvious apoptotic peak would immediately appear before diploid peak. Human soluble TRAIL protein can quickly kill MG-63 osteosarcoma cells selectively, and may have potential value for clinical treatment of osteosarcoma.