miRNA studies in in vitro and in vivo activated hepatic stellate cells

作     者：Gunter Maubach Michelle Chin Chia Lim Henry Yang 

作者机构：Institute of Bioengineering and Nanotechnology 31 Biopolis Way The Nanos #04-01 Singapore 138669 Singapore Institute of Experimental Internal Medicine Leipziger Strasse 44 Magdeburg 39120 Germany Institute of Bioengineering and Nanotechnology 31 Biopolis Way The Nanos #04-01 Singapore 138669 Singapore Bioinformatics Lab Singapore Immunology Network 8A Biomedical Grove Singapore 138648 Singapore 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2011年第17卷第22期

页      面：2748-2773页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Institute of Bioengineering and Nanotechnology (Biomedical Research Council  Agency for Science  Technology and Research  Singapore) 

主　　题：Hepatic stellate cells miRNA miR-146a Nuclear factor-κB 

摘      要：AIM: To understand which and how different miRNAs are implicated in the process of hepatic stellate cell (HSC) activation. METHODS: We used microarrays to examine the differential expression of miRNAs during in vitro activation of primary HSCs (pHSCs). The transcriptome changes upon stable transfection of rno-miR-146a into an HSC cell line were studied using cDNA microarrays. Selected differentially regulated miRNAs were investigated by quantitative real-time polymerase chain reaction during in vivo HSC activation. The effect of miRNA mimics and inhibitor on the in vitro activation of pHSCs was also ***: We found that 16 miRNAs were upregulated and 26 were downregulated significantly in 10-d in vitro activated pHSCs in comparison to quiescent pHSCs. Overexpression of rno-miR-146a was characterized by marked upregulation of tissue inhibitor of metalloproteinase-3, which is implicated in the regulation of tumor necrosis factor-α activity. Differences in the regulation of selected miRNAs were observed comparing in vitro and in vivo HSC activation. Treatment with miR-26a and 29a mimics, and miR-214 inhibitor during in vitro activation of pHSCs induced significant downregulation of collagen type Ⅰ transcription. CONCLUSION: Our results emphasize the different regulation of miRNAs in in vitro and in vivo activated pHSCs. We also showed that miR-26a, 29a and 214 are involved in the regulation of collagen type I mRNA.