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Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

作     者:Yuriy G.SHCKORBATOV Vladimir N.PASIUGA Elena I.GONCHARUK Tatiana Ph.PETRENKO Valentin A.GRABINA Nicolay N.KOLCHIGIN Dmitry D.IVANCHENKO Victor N.BYKOV Oleksandr M.DUMIN 

作者机构:Kharkiv National University Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine 

出 版 物:《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报(英文版)B辑(生物医学与生物技术))

年 卷 期:2010年第11卷第10期

页      面:801-805页

核心收录:

学科分类:0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主  题:Human cells Chromatin Heterochromatin Electromagnetic field Elliptical polarization 

摘      要:To investigate the influence of microwave radiation on the human fibroblast nuclei,the effects of three variants of electromagnetic wave polarization,linear and left-handed and right-handed elliptically polarized,were *** conditions were:frequency(f) 36.65 GHz,power density(P) at the surface of exposed object 1,10,30,and 100 μW/cm2,exposure time 10 *** fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic *** layer of medium that covered cells during microwave exposure was about 1 mm *** were stained immediately after irradiation by 2%(w/v) orcein solution in 45%(w/v) acetic *** were made at room temperature(25 °C),and control cell samples were processed in the same *** assessed heterochromatin granule quantity(HGQ) at 600× *** irradiation at the intensity of 1 μW/cm2 produced no effect,and irradiation at the intensities of 10 and 100 μW/cm2 induced an increase in *** intense irradiation induced more chromatin *** right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.

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