Coking wastewater increases micronucleus frequency in mouse in vivo via oxidative stress

作     者：Na Zhu Hongyan Li Guangke Li Nan Sang 

作者机构：College of Environment and Resource Center of Environmental Science and Engineering Shanxi University 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2013年第25卷第10期

页      面：2123-2129页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：supported by the National Natural Science Foundation of China (No.20977060) the Specialized Research Fund for the Doctoral Program of Higher Education(No.20121401110003) the Project for Science and Technology Development of Shanxi Province(No.20120313009-2) the Natural Science Foundation of Shanxi Province(No.2012021008-1) the Research Project Supported by Shanxi Scholarship Council of China(No.2011-013,2012-009) the Program for the Top Young and Middle aged Innovative Talents of Higher Learning Institutions of Shanxi 

主　　题：micronucleus oxidative stress coking wastewater mouse bone marrow 

摘      要：Coking wastewater has caused serious health risk in coal-producing areas of China, however its toxic effects have not been well understood. The genotoxicity induced by coking wastewater on mice in vivo and its possible oxidative mechanisms were investigated via observing the induction of micronuclei in polychromatic erythrocytes of mouse bone marrow, and subsequently determining the antioxidative enzyme activities （superoxide dismutase Cu, Zn-SOD, Se-dependent glutathione peroxidase, and catalase）, thiobarbituric acid reactive substance contents and protein carbonyl levels in brains and livers of mice. Results showed that the tested coking wastewater caused a significant increase of micronucleus frequencies in a concentration-dependent manner. Also, the sample increased lipid peroxidation and protein oxidation levels, which was accompanied by changes in antioxidative status. Interestingly, pre-treatment with an antioxidant （vitamin C） led to a statistical reduction in the micronucleus frequency caused by coking wastewater. This implies that coking wastewater induces evident genetic damage in mammalian cells, and exposure to polluted areas might pose a potential genotoxic risk to human beings; in the process, oxidative stress played a crucial role.