Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry

作     者：陈慕雁 Yang Hongsheng Shi Fangfang 

作者机构：Institute of Oceanology Chinese Academy of Sciences Qingdao 266071P.R.China Graduate University Chinese Academy of Sciences Beijing 100049 P.R.China 

出 版 物：《High Technology Letters》 (高技术通讯（英文版）)

年 卷 期：2007年第13卷第2期

页      面：221-224页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China (No.30170742) and National Key Foundational Research Project of China (No.G1999012012) 

主　　题：hemolymph anticoagulant solution TEM flow cytometry Chlamys farreri 

摘      要：Mixtures of hemolymph from Chlamys farreri with three different anticoagulant solutions were incu-bated for an hour in vitro, then the ultrastructural alterations of hemocytes were observed, and the aggre-gation rate was analyzed by using transmission electron microscropy and flow cytometry respectively. The results showed that Formula 3 （glucose 20.8 g L^-1; EDTA 20mM; sodium chloride 20 g L^-1; Tris-HCI 0.05M;pH 7.4）was the desirable anticoagulant solution for C. farreri hemocytes. Further phagocytosis assay showed that no obvious negative effect was given to the hemocyte phagocytic activity when using Formula 3 as the anticoagulant solution.