Construction of pcDNA3. 1 (+)tPA expression vector

作     者：ZHAO Yongbo Ll Yu ZHANG Guiyin 

作者机构：Department of Neurology TheSecond Affiliated HospitalHarbin Medical University Harbin 150086 Department of Neurology TheSecond Affiliated HospitalHarbin Medical University Harbin 150086 Department of Neurology TheSecond Affiliated HospitalHarbin Medical University Harbin 150086 

出 版 物：《中国临床神经科学》 (Chinese Journal of Clinical Neurosciences)

年 卷 期：2000年第8卷第Z1期

页      面：58-页

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

主　　题：expression vector plasminogen activator fibrinolytic activity genetic engineering recombinant vector molecular cloning plasmid DNA methods for method of 

摘      要：Objective To construct a new kind of recombinant vector containing human tissue-type plasminogen activator(t-PA) cDNA for studing the feasibility of enhancing fibrinolytic activity by transplantation of genetic engineering cells. Methods We recombinated human tPA cDNA with expression vector pcDNA3. 1 (+) by using the method of molecular cloning, sequenced the plasmid DNA, and cut the plasmid by using enzymes. Results The plasmid pcDNA3.1(+)tPA was divided into 5.4Kb and 2.0Kb segments respectively by using Kpn 1 and Xba I, into 78bp, 414bp, 622bp, 2.0Kb, and4.2Kb segments respectively by tsing Pst l, into 472bp and 6.9Kb segments respectively by using EcoR I. Sequencing result showed that it is the whole human tPA cDNA. Conclusion The new kind of recombinant expression vector could serve as new tools and methods for preventing thrombogenic diseases.