Influence of location-dependent protuberance damage on cell viability

作     者：YANG HaiFeng ZHOU Ming DI JianKe ZHAO EnLan YANG PeiFang GONG AiHua SUN XiangLan 

作者机构：Photonics Fabrication Science Center Jiangsu University Zhenjiang 212013 China School of Medical Science and Laboratory Medicine Jiangsu University Zhenjiang 212013 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2009年第54卷第7期

页      面：1260-1266页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by National High Technology Research & Development Program of China (Grant No. 2006AA04Z307) Author of National Excellent Doctoral Dissertation of PR China (Grant No. 2006039) National Natural Science Foundation of China (Grant No. 50775104) Natural Science Foundation of Jiangsu Province (Grant No. BK2006507) Jiangsu Provincial Research Innovation Program for College Graduates (Grant No. CX07B_086z) 

主　　题：细胞活力 位置依赖 细胞形态变化 飞秒激光 激光诱导损伤 细胞死亡 激光手术 细胞存活率 

摘      要：The influence of femtosecond laser-induced damages on viability of olfactory ensheathing cells (OECs) is investigated. Several cytokinetic processes including cellular damage, recovery and death are discussed. Using femtosecond laser with the power of 100 μW and cutting speed of 2 μm/s, we cut the cellular protuberance with smaller diameter twice in different locations, and then observe the viability of the damaged cells. Under the same conditions, the root of protuberance with larger diameter is cut six times to observe changes of cellular shape. Whether the damage is located in the end, middle or root of protuberance with smaller diameter, the cell viability can recover within 3 h. When the damage is located in the root of protuberance with larger diameter, the damaged cell will die in the way of oncosis. Cytokinetic phenomena including intracellular high Ca2+ concentration, cellular morphologic change, recovery and oncosis are discussed. Meanwhile, high Ca2+ concentration is observed after femtosecond laser surgery. Therefore, femtosecond laser surgery is an important tool for establishing cell damage model and studying cytokinetics.