Expression of p27,cyclin E and cyclin A in hepatocellular carcinoma and its clinical significance

作     者：Qi Zhou Qiang He Li-Jian Liang, Department of Hepatobilliary Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guandong Province, China 

作者机构：Department of Hepatobilliary Surgery the First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guandong Province China. 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第11期

页      面：2450-2454页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：细胞周期调节蛋白E 细胞周期调节蛋白A p27 肝细胞癌 基因表达 临床意义 肿瘤侵袭 肿瘤转移 

摘      要：AIM: TO investigate the expression of p27, cyclin E andcyclin A in hepatocellular carcinoma (HCC) and its potential clinical ***: Expression of p27, cyclin E and cyclin A in 45 HCC specimens and 30 adjacent noncancerous lesions obtained from 45 patients during surgery was examined by immunohistochemical SABC assay. The diameter of tumor ranged from 1 cm to 19 cm (d≤5 cm, 9 samples; 5 cm10 cm, 17 samples). The tumors were graded according to the criteria described by EdmondsonSteiner: well-differentiated HCC group (Grade Ⅰ+Ⅱ), 26 samples; poorly-differentiated HCC group (Grade Ⅲ+Ⅳ),19 samples. According to the clinical-pathologic features,19 samples were poorly encapsulated, 15 samples had portal invasion of cancer, 11 samples had extrahepatic metastasis,and 12 samples had intrahepatic metastasis. All of the samples were classified as the invasive and metastatic group,while the remaining was classified as the non-invasive and non- metastatic ***: The average labeling index (LI) of p27 in HCC lesions was significantly higher than that in adjacent noncancerous lesions (45.87±14.21 vs33.77±12.92, t=3.745,P0.001). The LI of p27 was associated with differentiation,invasiveness and metastasis of the tumors (34.46±12.29 vs 52.80±11.36, t=5.17; 41.42±12.86 vs51.44±14.10, t=2.48;P0.05). Cyclin E was overexpressed in 16 cases (35.6 %)while cyclin A was overexpressed in 21 cases (46.7 %) in HCC lesions. No overexpression of cyclin E or cyclin A could be observed in adjacent non-carcinoma lesions and normal liver tissues. The overexpressions of cyclin E and cyclin A were correlated with differentiation, tumor thrombus,invasiveness and metastasis (P0.05). Expression of cyclin E was significantly correlated with expression of cyclin A (r=0.329, P0.05). The LI of p27 was significantly decreased in cyclin E, cyclin A positive groups (40.33±11.91 vs 49.50±13.76, t=3.05; 38.86±11.19 vs 52.57±12.62,t=3.89; P0.05).CONCLUSION: p27,