Study on Saccharification of Saccharum spontaneum L. by Sulphuric Acid Hydrolysis
Study on Saccharification of Saccharum spontaneum L. by Sulphuric Acid Hydrolysis作者机构：College of AgronomyHainan University Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural Sciences/Key Laboratory for Utilization of Tropical Crop Germplasm Resources Ministry of Agriculture
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2014年第3卷第6期
页 面：8-13页
基 金：Supported by Germplasm Collection and Collaborative Research in Central and South America(2011DFB31690) "948"Project of Ministry of Agriculture of China"Introduction of Major Tropical Crop Germplasm Resources" Project of Species Resource Protection of the Ministry of Agriculture"Preservation of Tropical Forage Germplasm Resources" Project of Tropical Crop Germplasm Resource Protection of the Ministry of Agriculture(No.12RZZY-09) Modern Agricultural Talent Support Program of the Ministry of Agriculture"Innovation and Utilization of Tropical Forage"
主 题：Saccharum spontaneum L. Acid hydrolysis Two-step acid hydrolysis
摘 要：In order to improve the saccharification rate by acid hydrolysis in the technique for production of ethanol from biomass, in this study, Saccharum spontaneum L. was used as the experimental material to investigate the rules and technological conditions for two-step acid-hydrolysis saccharification by single factor experiment and orthogonal experiment. According to the results, concentrated sulfttric acid hydrolysis was the first step, with the liquid-solid ratio of 42： 1, sulfuric acid concentration of 70%, hydrolysis time of 20 rain, and hydrolysis temperature of 55 ℃ ; dilute sulphuric acid hydrolysis was the second step, with the liquidsolid ratio of 115： 1, sulfuric acid concentration of 5.5%, hydrolysis time of 155 min, and hydrolysis temperature of 100℃. In two-step acid hydrolysis process, the yield of reducing sugar was 48.78%. The results indicated that two-step acid-hydrolysis saccharification required mild conditions, simple operation and low cost, and led to high reducing sugar yield, exhibiting a broad application prospect.
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