Cancer-derived VEGF plays no role in malignant ascites formation in the mouse

作     者：Bayasi Guleng Keisuke Tateishi Fumihiko Kanai Amarsanaa Jazag Miki Ohta Yoshinari Asaoka Hideaki Ijichi Yasuo Tanaka Jun Imamura Tsuneo Ikenoue Yasushi Fukushima Keita-Morikane Makoto Miyagishi Kazunari Taira Takao Kawabe Masao Omata 

作者机构：Department of GastroenterologyGraduate School of Medicine University of Tokyo 7-3-1 HongoBunkyo-ku Tokyo 113-8655 Japan Clinical Research CenterUniversity of Tokyo Hospital 7-3-1 Hongo Bunkyo-ku Tokyo113-8655 Japan Infectious Disease Surveillance Center NationalInstitute of Infectious Diseases 1-23-1 Toyama Shinjuku-ku Tokyo162-8640 Japan Department of Chemistry andBiotechnology Graduate School of Engineering University of TokyoTokyo 113-8656 Japan 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2005年第11卷第35期

页      面：5455-5459页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：血管内皮生长因子 癌症 恶性腹水 并发症 小鼠 动物实验 

摘      要：AIM: Vascular endothelial growth factor (VEGF) is a potent mediator of peritoneal fluid accumulation following tumor progression. This study investigated the role of VEGF secreted by cancerous cells in the formation of malignant ***: VEGF expression was eliminated byknockdown in the pancreas cancer cell-line PancO2 using vector-based short-hairpin type RNA interference (RNAi).Malignant ascites formation in the mouse was analyzed by intraperitoneal injection of PancO2 cells expressing VEGF or with expression ***: The VEGF knockdown PancO2 cell was successfully established. Knockdown of VEGF did not affect cancer cell proliferation in vitro or in vivo. The volume of ascites following peritoneal expansion of the tumor in VEGF knockdown cells and control cells did not differ statistically in this in vivo study. Moreover, the VEGF concentration in the ascites did not differ ***: Malignant ascites formation might be mediated by VEGF production in noncancerous tissues,such as stromal compartments. An anti-VEGF strategy against malignant ascites could be applied to various tumors regardless of whether they secrete VEGF.