Effects of the Application of Different Concentrations of NaN3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley （Hordeum vulgate L.） Seedlings

作     者：Ali Irfan ILBAS Yasemin EROGLU Halil Erhan EROGLU 

作者机构：Department of Biology Faculty of Yozgat Arts and Science Erciyes University 66100 Yozgat Turkey 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2005年第47卷第9期

页      面：1101-1106页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：Supported by the Department of Scientific Researches of Erciyes University (FBA.03.31).Publication of this paper is supported by the National Natural Science Foundation of China (30424813) and Science Publication Foundation of the Chinese Academy of Sciences 

主　　题：Hordeum vulgare mitotic index NAN3. 

摘      要：Sodium azide （NAN3） has been used in many biological studies as a mutagen. In the present study, the morphological and cytogenetic effects of NaN3 on barley （Hordeum vulgare L.） seedlings were investigated. Seeds of barley were treated with different concentrations of NaN3 （0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mmol/L） and applied for different periods of time （3 and 4 h）. Parameters investigated, except for the mitotic index, were determined on Days 7 and 14. Increasing concentrations of NaN3 affected germination rates on Days 7 and 14 following application for 3 and 4 h. Although the length of the roots and leaves was affected by treatment with NaN3 on Day 14 of the germination period, coleoptile length was affected by NaN3 treatment on Day 7. Increasing concentrations of NaN3 and increased treatment period decreased the mitotic index compared with the untreated control. The inhibitory effects of NaN3 on the mitotic index indicate that NaN3 can have genotoxic and mutagenic effects on barley seedlings.