Wuling powder alleviates depressive-like behavior by attenuating endoplasmic reticulum stress in the mouse hippocampus
乌灵菌粉通过减弱小鼠海马内质网应激缓解抑郁样行为作者机构：Department of PharmacologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China
出 版 物：《Journal of Chinese Pharmaceutical Sciences》 (中国药学（英文版）)
年 卷 期：2024年第33卷第9期
页 面：783-794页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：National Natural Science Foundation of China(Grant No.81100801 and 81050025) the foundation of Zhejiang Jolly Pharmaceutical Co.,Ltd
主 题：Depression Wuling powder Endoplasmic reticulum stress Unfolded protein response
摘 要：The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in *** powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,has demonstrated efficacy in alleviating depressive *** purpose of this research was to explore the antidepressant properties of Wuling powder and its basic molecular effects,particularly regarding alterations in ER stress.A model of social defeat stress was created by introducing a mouse to the cage of an unfamiliar,hostile mouse for intervals of 5–10 min daily over a span of 10 ***,the mice received oral doses of Wuling powder for 2 *** social approach-avoidance assay was employed to evaluate signs of depression-like ***,protein and gene expressions linked to ER stress triggered by social defeat were analyzed through Western blotting analysis and quantitative real-time *** behavioral tests indicated that Wuling powder ameliorated behaviors associated with depression due to social defeat *** with Wuling powder markedly reduced the increased levels of the 78-k Da glucose-regulated protein and protein disulfide isomerase caused by social defeat *** also diminished the expression of inositol-requiring enzyme 1α(IRE1α)and spliced X box-binding protein-1(s XBP1)at the protein and m RNA ***,Wuling treatment notably decreased the levels of phosphorylated eukaryotic initiation factor 2 alpha kinase(P-e IF2α),activating transcription factor 4(ATF4)and C/EBP homologous protein(CHOP),simultaneously enhancing the ratio of B-cell lymphoma 2(Bcl-2)to Bcl-2-associated X protein(Bax).These results suggested that Wuling powder could alleviate ER stress and inhibit cell apoptosis in the hippocampus by inhibiting protein translation and synthesis,thereby attenuating depressive-like behavior.
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