Tumor Interstitial Fluid and Postoperative Recurrence of Tumors: an Experimental Study for Verifying Hypothesis of "Tumor-phlegm Microenvironment"

作     者：孙大志 巨大维 何金 陆烨 武峰 李畅 魏品康 

作者机构：Department of Traditional Chinese Medicine Shanghai Changzheng Hospital the Second Military Medical University Department of Laboratory Digniostics Shanghai Changzheng Hospital the Second Military Medical University Department of Pathology Shanghai Changzheng Hospital the Second Military Medical University 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2010年第16卷第5期

页      面：435-441页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by National Natural Science Foundation (Major Research Project, No. 90709044) Shanghai Changzheng Hospital "Three Major, Three Merit" Project Fund 

主　　题：tumor interstitial fluid muddy phlegm interleukin-8 tumor recurrence 

摘      要：Objective： To explore a method of extracting tumor interstitial fluid （TIF） which is similar to muddy phlegm in Chinese medicine （CM）, interleukin-8 （IL-8） in concentration was taken as the representative of the content of TIF, analyzed in the extracted TIF and the original tumor tissue, and examined to see whether TIF has an interfering effect on tumor recurrence. Methods： Tumor tissue was ground, centrifuged, and filtered for intercellular substances. Tumor-bearing Kunming S180 mice were raised for 21 days and then the tumors were removed to observe the influence of intervention with TIF, normal saline （NS） and a blank control on tumor recurrence. Results： The content of IL-8 in the filtered and unfiltered tumor tissue was not significantly different （P0.05）. Postoperative tumor recurrence in TIF intervention group was significantly higher than that in the NS intervention and control groups （60%, 12/20 vs. 20%, 4/20 vs. 15%, 3/20, χ2=11.058, P0.01）. Tumor cells grew vigorously and infiltrated to muscular tissue in TIF intervention group. Large numbers of tumor cells were seen necrotic in the NS intervention group, and small numbers of tumor cells were seen necrotic in the blank control group. Conclusions： TIF can be effectively extracted by the means described. It does not contain tumor cells, but its contents such as IL-8 may stimulate tumor cell growth and promote postoperative tumor recurrence, which provided preliminary experimental basis for hypothesis of ＂tumor-phlegm microenvironment＂.