The response of intestinal stem cells and epithelium after alemtuzumab administration

作     者：Qiurong Li Qiang Zhang Chenyang Wang Shaojun Jiang Ning Li Jieshou Li 

作者机构：Department of SurgeryJinling HospitalNanjing University School of MedicineNanjingChina Department of PathologyJinling HospitalNanjingChina 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2011年第8卷第4期

页      面：325-332页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Basic Research Program(973 Program)in China(nos.2009CB522405 and 2007CB513005) the Key Project of National Natural Science Foundation in China(30830098) the National Natural Science Foundation in China(81070375) the Scientific Research Fund in Jiangsu Province(BK2009317) the National Key Project of Scientific and Technical Supporting Programs Funded by the Ministry of Science and Technology of China(2008BAI60B06) the Military Scientific Research Fund(0603AM117) 

主　　题：alemtuzumab intestinal stem cells intestinal stem cell marker Musashi-1 Lgr5 

摘      要：Qiurong Li1,Qiang Zhang1,Chenyang Wang1,Shaojun Jiang2,Ning Li1,Jieshou Li1 Intestinal stem cells may have important roles in the maintenance of epithelial integrity during tissue *** is a humanized anti-CD52 lymphocytic antibody that is increasingly being used to induce immunosuppression;intestinal barrier function is impaired during treatment with *** investigated the response of intestinal stem cells to epithelial damage resulting from alemtuzumab *** epithelial cell loss and abnormal Paneth cell morphology were found following a single dose of *** animals receiving alemtuzumab exhibited increased apoptosis in the villi 3 days after alemtuzumab treatment and in the crypt on day 9,but apoptosis was scarce on day *** assessed expression of Musashi-1-and Lgr5-positive stem cells following alemtuzumab *** numbers of cells staining positive for both Musashi-1 and Lgr5 were found in the stem cell zone after alemtuzumab treatment for 3 and 9 *** data indicated that the epithelial cells were injured following alemtuzumab treatment,with the associated expansion of intestinal stem *** alemtuzumab treatment for 35 days,the numbers of intestinal epithelial cells and intestinal stem cells returned to *** study suggests that alemtuzumab treatment induced the increase in stem cells,resulting in the availability of more enterocytes for repair.