Arabidopsis co-chaperonin CPN20 antagonizes Mg-chelatase H subunit to derepress ABA-responsive WRKY40 transcription repressor

作     者：ZHANG XiaoFeng JIANG Tao YU YongTao WU Zhen JIANG ShangChuan LU Kai FENG XiuJing LIANG Shan LU YanFen WANG XiaoFang ZHANG DaPeng 

作者机构：MOE Systems Biology and Bioinformatics Laboratory School of Life Sciences Tsinghua University 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2014年第57卷第1期

页      面：11-21页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Key Basic Research Program of China(2012CB114302) National Natural Science Foundation of China(90817104 and 31170268) Ministry of Agriculture of China(2013ZX08009-003) 

主　　题：co-chaperonin CPN20 Mg-chelatase H subunit WRKY40 ABA signalling 

摘      要：Our previous study demonstrated that a chloroplast co-chaperonin 20(CPN20),one of the interaction partners of the magnesium-protoporphyrin IX chelatase H subunit(CHLH/ABAR),negatively regulates ABA signaling at the same node with ABAR but upstream of WRKY40 transcription repressor in Arabidopsis *** the present experiment,we showed that ABA directly inhibits the ABAR-CPN20 interaction,and also represses expression of CPN20,which depends on ***20 inhibits ABAR-WRKY40 interaction by competitively binding to *** downregulates,but CPN20 upregulates,WRKY40 *** cpn20-1 mutation induces downregulation of WRKY40,and suppresses the upregulated level of WRKY40 due to the cch mutation in the ABAR ***-induced repressive effect of the WRKY40 gene is strengthened by downregulation of CPN20 but reduced by upregulation of *** with our previously reported genetic data,we provide evidence that CPN20 functions through antagonizing the ABAR-WRKY40 coupled pathway,and ABA relieves this pathway of repression by inhibiting the ABAR-CPN20 interaction to activate ABAR-WRKY40 interaction.