RISE Method Based on Rare Allele Infusion and Sanger Sequencing Estimation: A Simple, Cheap, and Efficient Method for Detecting Transgene Copy Number in Rice

作     者：LIU Tingchang HUANG Lifang LIU Peng CUI Yanchun CHEN Caiyan MAO Donghai 

作者机构：Key Laboratory of Agro-Ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2024年第31卷第5期

页      面：499-502,I0014-I0020页

核心收录：

学科分类：08[工学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the Natural Science Foundation of Hunan Province for Distinguished Young Scholars, China (Grant No.2021JJ10041) the Biological Breeding-National Science and Technology Major Project, China (Grant No.2023ZD04072) the Hainan Yazhou Bay Seed Laboratory and National Seed Group, China (Grant Nos.B23CQ15HP and B23YQ1517) 

主　　题：Sanger cost Det 

摘      要：Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.