αB-crystallin mini-peptides support corneal healing in vitro and in vivo in rabbit model
作者机构：Department of Veterinary Surgery&RadiologyWest Bengal University of Animal and Fishery SciencesKolkata 700037India CSIR-IICBKolkata 700032India
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2024年第17卷第10期
页 面：1772-1779页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金：Supported by the DST Nano-mission,Govt of India,Grant No DST No.SR/NM/NS-1067/2016 Facilities were provided by the West Bengal University of Animal&Fishery Sciences and CSIR-IICB for conducting this research
主 题：corneal healing αB-crystallin cytoprotection neuroprotection
摘 要：AIM:To evaluate if topical use of αB-crystallin minipeptides supports corneal healing following flap ***:Cultured corneal cells were treated with fluorescent taggedαB-crystallin mini-peptides to assess its *** corneal cells pre-treated with or without the mini-peptides were exposed to H_(2)O_(2) and cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)*** of neurites of cultured trigeminal neurones was examined following treatment either withαB-crystallin mini-peptides or *** trigeminal neurones were pre-treated either with αB-crystallin mini-peptides or crystallin protein and exposed to H_(2)O_(2) and presence of beading in the dendrites and axons was *** flap surgery was conducted on rabbit cornea and treated topically either withαB-crystallin peptide(0.5 mg/mL thrice daily for 14d)or phosphate-buffered saline(PBS).Corneal healing was evaluated under slit-lamp biomicroscope,mRNA expression of inflammatory cytokines were assessed and the corneas were evaluated by ***:Internalization ofαB-crystallin mini-peptides was ascertained by the detection of fluorescence within the corneal *** MTT assay revealed that treatment withαB-crystallin mini-peptide reduced cell death induced by H_(2)O_(2) *** mini-peptides did not influence the elongation of trigeminal neurites,but significantly(P0.05)reduced beading in the *** rabbit eye,the treated corneas showed reduced hyper-reflective zones(P0.05)and suppression in the expression of inflammatory *** examination also revealed reduction of inflammatory response in treated ***:TheαB-crystallin mini-peptides restrict the damage to corneal cells and neurons and aids in corneal healing.
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