Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats
Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats作者机构:Qingdao Research Center of Microecology-Engineering Technology Jiaonan 266400 Shandong Province China BRITE/Department of Pharmaceutical Sciences North Carolina Central University Durham NC27707 United States Pathology Department of People's HospitalJiaonan 266400 Shandong Province China Qingdao East Sea Pharmaceutical Co. LtdJiaonan 266400 Shandong Province China
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2009年第15卷第15期
页 面:1821-1828页
核心收录:
学科分类:1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金:The Model Subject of High-Tech Industrializing Conversion of Series Microecological New Drugs National Development and Reform Commission High-Tech 2078
主 题:Clostridium butyricum Interleukin-23 Tumor necrosis factor-m Calcitonin gene relatedpeptide Colitis
摘 要:AIM: To evaluate the therapeutic effects of a probiotic supplement (Clostridium butyricum, CGMCC0313 ) in a chemically-induced rat model of experimental colitis. METHODS: An experimental ulcerative colitis model was established by rectal injection of oxazolone into the colon of 40 Wistar rats randomly divided into four groups. The positive control group was sacrificed 3 d after colitis onset. The remaining groups were fed daily with either 2 mL of C. butyricum (2.3 × 10^11 CFU/L), 2 mL of mesalamine (100 g/L), or 1 mL of sodium butyrate (50 mmol/L) for 21 d. The animals' body weight, behavior, and bowel movements were recorded weekly. After sacrifice, visual and microscopic observations of pathological changes of colon tissue were made, body weight and wet colon mass index were measured and recorded, and serum levels of interleukin-23 (IL-23) and TNF-α were measured using ELISA. Expression of calcitonin gene-related peptide in colon tissue was measured by RT-PCR. Finally, changes in rat intestinal microflora status were measured in all *** the serum levels of both IL-23 and tumor necrosis factor-α (TNF-α) with similar or even better efficiency than that of mesalamine or sodium butyrate. The rat intestinal flora appeared to recover more quickly in the group treated with C. butyricum than in the mesalamine and sodium butyrate groups. Finally, we found that the expression level of calcitonin gene related peptide was elevated in colon tissue in the sodium butyrate treated group but not in the C. butyricum or mesalamine treated groups, indicating a sensitization of colon following sodium butyrate treatment. CONCLUSION: In our experimental colitis model, treatment with C. butyricum CGMCC0313, a probiotic supplement, is at least as efficient as treatment with mesalamine.