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Effects of Zn^(2+) on root growth,cell division,and nucleoli of Allium cepa L.

Effects of Zn ̄(2+) on root growth,cell division, and nucleoli of Allium cepa L.

作     者:Liu Donghua Jiang Wusheng Wang Chunli (Department of Biology, Tianjin Normal University,Tianjin 300074,China)Zhai Lin (Xinhua High School , Tianjin 300204,China) 

出 版 物:《Journal of Environmental Sciences》 (环境科学学报(英文版))

年 卷 期:1996年第8卷第1期

页      面:21-27页

核心收录:

学科分类:083002[工学-环境工程] 0830[工学-环境科学与工程(可授工学、理学、农学学位)] 08[工学] 

主  题:Allium cepa mitosis chromosome aberration nucleoli silver staining technique. 

摘      要:The effects of different concentrations of Zn ̄(2+)ion on root growth,cell division,and nucleoli of Allium cepa were studied. The test Zn ̄(2+) ion concentration was made up from zinc sulphate (ZnSO4. 7H2O) ranging from 10 ̄(-7) to 10 ̄(-2) mol/L. The solutions were prepared in tap water (pH =6. 5).The results indicated that Zn ̄(2+) could obviously inhibit root growth at concentrations from 10 ̄(-4)to 10 ̄(-2) mol/*** treated with zinc sulphate showed the presence of c-mitosis, anaphase bridges,including sticky and fluidized bridges (at 10 ̄(-3) to 10 ̄(-2) mol/L) , chromosome stickiness, irregularly shaped nuclei, broken nuclei and micronuclei. A toxicity effect was also observed on the nucleoli using silver staining technique after 48h of treatment with 10 ̄(-4)to 10 ̄(-2) mol/L Zn ̄(2+), e. g,the nucleolar particulate material scattered around the nucleoli in the nucleus of root tip cells.

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