Establishment of a Lateral Flow Dipstick Detection Method for Influenza A Virus Based on CRISPR/Cas12a System
作者机构：Department of Clinical LaboratoryZhumadian Central HospitalZhumadian CityHenan ProvinceChina Laboratory of VirologyBeijing Key Laboratory of Etiology of Viral Diseases in ChildrenCapital Institute of PediatricsBeijingChina National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina
出 版 物：《China CDC weekly》 (中国疾病预防控制中心周报（英文）)
年 卷 期：2024年第6卷第37期
页 面：946-952,I0008页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
主 题：prevention diagnosis specificity
摘 要：Objective:This study aimed to develop a rapid,visual PCR-CRISPR/Cas12-LFD method for detecting influenza A by utilizing the conserved region of the matrix protein ***:We crafted universal degradation primers and clustered regularly interspaced short palindromic repeats RNA(CRISPR RNA,crRNA)targeting the conserved matrix protein gene of the influenza virus(IFV),integrated with lateral flow dipstick(LFD)*** new PCR-CRISPR/Cas12-LFD approach was designed to determine its sensitivity and specificity through the analysis of various clinical samples collected in ***:The developed nucleic acid assay for influenza A viruses(IAV)demonstrated a sensitivity of 10 copies/μL without cross-reactivity with other respiratory *** of 82 clinical samples showed high concordance with results from fluorescent Polymerase Chain Reaction(PCR),achieving a kappa value of ***:A highly sensitive and specific PCRCRISPR/Cas12-LFD method has been successfully established for the detection of influenza A,offering a robust tool for its diagnosis and aiding in the prevention and control of this virus.
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