DNA hypermethylation of COL4A1 in ultraviolet-Binduced age-related cataract models in vitro and in vivo
作者机构：Department of OptometryXi’an Medical UniversityXi’an 710021Shaanxi ProvinceChina Department of Ophthalmologythe Second Affiliated Hospital of Xi’an Medical UniversityXi’an 710038Shaanxi ProvinceChina Department of OphthalmologyXijing 986 HospitalFourth Military Medical UniversityXi’an 710054Shaanxi ProvinceChina
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2024年第17卷第10期
页 面：1791-1799页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by the Shaanxi Provincial Department of Science and Technology Agency Project(No.2022SF-502) Xi’an Medical University Scientific Research Capacity Improvement Project(No.2022NLTS104) The Fifth Batch of Key Disciplines of Xi’an Medical University(No.medical technology12202306) Yunzhen Optometry Special Fund(No.2021HXZR10) Innovation and Entrepreneurship Training Program for College Students(No.S202211840043)
主 题：human lens epithelium cells age-related cataract COL4A1 hypermethylation ultraviolet-B rat
摘 要：AIM:To explore the DNA methylation of COL4A1 in ultraviolet-B(UVB)-induced age-related cataract(ARC)models in vitro and in ***:Human lens epithelium B3(HLEB3)cells and Sprague Dawley rats were exposure to UVB *** MTT assay was utilized to evaluate cell *** cytometry was employed for analysis of cell apoptosis and cell ***4A1 expression in HLEB3 cells and anterior lens capsules were assessed using Western blot and reverse transcription-polymerase chain reaction(RTPCR).The localization of COL4A1 in HLEB3 cells was determined by *** methylation status of CpG islands located in COL4A1 promoter was verified using bisulfite-sequencing PCR(BSP).DNMTs and TETs mRNA levels was examined by ***:UVB exposure decreased HLEB3 cells proliferation,while increased the apoptosis rate and cells were arrested in G0/G1 ***4A1 expression was markedly inhibited in UVB treated cells compared to the *** status was detected in the CpG islands within COL4A1 promoter in HLEB3 cells subjected to UVB *** of DNMTs including DNMT1/2/3 were elevated in UVB treated HLEB3 cells compared to that in the controls,while expressions of TETs including TET1/2/3 showed the opposite *** from the UVB treated rat model further confirmed the decreased expression of COL4A1,hypermethylation status of the CpG islands at promoter of COL4A1 and abnormal expression of DNMT1/2/3 and TET1/2/in UVB exposure ***:DNA hypermethylation of COL4A1 promoter CpG islands is correlated with decreased COL4A1 expression in UVB induced HLEB3 cells and anterior lens capsules of rats.
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