Modified streptozotocin-induced diabetic model in rodents

作     者：Anton Lennikov Farris ElZaridi Menglu Yang Anton Lennikov;Farris ElZaridi;Menglu Yang

作者机构：Department of OphthalmologySchepens Eye Research InstituteMass Eye and EarHarvard Medical SchoolBostonMassachusettsUSA 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2024年第7卷第5期

页      面：777-780页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Schepens Eye Research Institute of Massachusetts Eye and Ear Department of Ophthalmology Massachusetts Lions Charitable Foundation U.S. Department of Defense, DOD, (HT9425‐23‐1‐1045) U.S. Department of Defense, DOD 

主　　题：hyperglycemia non- fasting streptozotocin Type 1 diabetes mellitus 

摘      要：Streptozotocin(STZ)-induced type I diabetes mellitus(DM)models have been pivotal in diabetes research due to their ability to mimic the insulin-dependent hyperglyce-mia akin to human type I ***,these models often suffer from poor induction rates and low survival post-STZ induction,especially in long-term experi-ments,necessitating insulin supplementation,which introduces additional variables to *** address this,we present a novel modification to the STZ-induced DM model in C57BL/6J mice to improve survival rates without insulin *** method involves non-fasting,low-dose STZ injections dissolved in pH-neutral phosphate buffer saline instead of acidic sodium citrate buffer,administered over 5 *** observed hyperglycemia induction in 94.28%of mice within a week post-injection,with stable high blood glucose levels,stable body weight,and minimal mortality up to 21 ***,omitting 10%sucrose in water and fasting did not affect hyperglycemia *** findings suggest that the modified protocol not only decreases the experimental effort of the researchers,but reduces animal stress and mortality,thus enhancing experimental outcomes and animal *** opti-mizing the STZ-induced DM model in C57BL/6J mice,our study provides a valuable resource for researchers aiming to study diabetes and its complications while mini-mizing experimental variability and animal usage.