Effect of Different Disinfection Methods on Calluses of Amorphophallus albus ***&***

作     者：CHEN Xu-yan LU Yi ZHENG Lin-lin FENG Yu-qian HAO Guo-qian 陈绪言;陆毅;郑林林;冯渝茜;郝国歉

作者机构：Faculty of AgricultureForestry and Food EngineeringYibin UniversityYibin 644000PRC 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2024年第25卷第2期

页      面：26-32页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：国家自然科学基金(31700164) 中央引导地方科技发展专项资金项目(2022ZYD02) 四川省大学生创新创业训练计划项目(S202310641176X) 国家级大学生创新创业训练计划项目(202410641048) 

主　　题：Amorphophallus albus Callus Anatomical observation Disinfection methods 

摘      要：This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation *** calluses induced from buds of *** were disinfected with benzalkonium bromide,mercury bichloride,sodium hypochlorite,and ethanol,respectively,for different time *** growth of calluses was compared between different treatments and observed in detail by *** results showed that the disinfection with 0.1% mercury bichloride for 5 min and 75% ethanol for 5 s had mild effect on the callus,with the lowest browning rates of 16.32% and 15.46% and the lowest vitrification rates of 3.24% and 9.72%,respectively,after 20 *** disinfection with benzalkonium bromide and sodium hypochlorite had strong effect on the callus cells,with the lowest browning rate of 50.69% and 25.12% and the lowest vitrification rates of 28.47% and 28.43%,*** cytological observation result showed that the calluses disinfected with ethanol and mercury bichloride showcased compact structures,while those disinfected with sodium hypochlorite and benzalkonium bromide exhibited loose *** sum up,it is recommended that 75% ethanol or 0.1% mercury bichloride can be used for disinfection in the tissue culture and rapid propagation of ***,while the use of benzalkonium bromide and sodium hypochlorite should be avoided as much as possible.