An allelic variation in the promoter of the LRR-RLK gene, qSS6.1, is associated with melon seed size
作者机构：College of Horticulture Henan Agricultural University Henan Engineering Research Center for Cucurbit Germplasm Enhancement and Utilization International Joint Laboratory of Henan Horticultural Crop Biology
出 版 物：《Journal of Integrative Agriculture》 (农业科学学报(英文版))
年 卷 期：2024年第10期
页 面：3522-3536页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：the Henan Special Funds for Major Science and Technology,China (221100110400) the Henan Scienti?c and Technological Joint Project for Agricultural Improved Varieties,China (2022010503) the National Natural Science Foundation of China (31902038 and 32072564)
摘 要：Seed size is an important agronomic trait in melons that directly affects seed germination and subsequent seedling ***, the genetic mechanism underlying seed size in melon remains *** the present study,we employed Bulked-Segregant Analysis sequencing （BSA-seq） to identify a candidate region （～1.35 Mb） on chromosome 6 that corresponds to seed *** interval was confirmed by QTL mapping of three seed sizerelated traits from an F2population across three *** mapping region represented nine QTLs that shared an overlapping region on chromosome 6, collectively referred to as *** InDel markers were developedintheqSS6.1 region, narrowing it down to a 68.35 kb interval that contains *** variation analysis of the eight genes identified a SNP with a C to T transition mutation in the promoter region of MELO3C014002, a leucine-rich repeat receptor-like kinase （LRR-RLK） *** mutation affected the promoter activity of the MELO3C014002 gene and was successfully used to differentiate the large-seeded accessions （C-allele） from the small-seeded accessions （T-allele）.qRT-PCR revealed differential expression of MELO3C014002 between the two parental *** predicted protein has typical LRR-RLK family domains, and phylogenetica***, these findings suggest MELO3C014002 as the most likely candidate gene involved in melon seed size *** results will be helpful for better understanding the genetic mechanism regulating seed size in melons and for genetically improving this important trait through molecular breeding pathways.
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