Huoxue Tongjiang decoction-resisted reflux esophagitis by activation stem cell factor/c-kit/interstitial cell of cajal pathway and regulating the T-helper 17/regulatory T-cells balance in rats
作者机构：Division of GastroenterologyHospital of Integrated Chinese and Western MedicineTianjin University of Traditional Chinese MedicineTianjin 300000China Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM RepairHospital of Integrated Chinese and Western MedicineTianjin 300000China Institute of Integrative Medicine for Acute Abdominal DiseasesHospital of Integrated Chinese and Western MedicineTianjin 300000China
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2024年第9卷第12期
页 面：1-12页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：supported by the National Natural Science Foundation of China(No.81573737 and 82074213) the science foundation of Tianjin Municipal Health Bureau(No.2023169 and 2021045) the Tianjin Municipal Health Commission Science and Technology Project(No.TJWJ2022QN057)
主 题：Huoxue Tongjiang decoction reflux esophagitis SCF/c-kit/ICC pathway Th17/Treg cell esophageal motility mucosal inflammation
摘 要：Background:Huoxue Tongjiang decoction(HXTJD)is an effective prescription for treating reflux esophagitis(RE).We investigated the effects of HXTJD on esophageal motility and mucosal inflammation in a rat RE ***:Chemical composition of HXTJD was analyzed by ultrahigh-performance liquid chromatography Q-Orbitrap mass spectrometry(MS).The change rates of mean contraction tension forces,mean amplitudes,and mean frequencies for the lower esophageal sphincter(LES)were recorded using the isolated tissue bath system,mechanical tension transducer,and PowerLab physiological *** weighing the stomach,the phenol red labeling method was used to measure the gastric emptying *** LES ultrastructure was observed through transmission electron *** and western blotting were used to detect the number of interstitial cells of Cajal(ICC)and the expression levels of c-kit protein,connexin43(Cx43),and stem cell factor(SCF).Flow cytometric analysis and enzyme-linked immunosorbent assay were conducted to detect the percentages of T helper 17(Th17)cells and regulatory T(Treg)cells and the serum concentrations of interleukin 6(IL-6),interleukin 17(IL-17),and interleukin 10(IL-10)in the ***:We identified 28 chemical constituents in *** esophageal motility,we revealed that HXTJD increased the mean contraction tension forces,mean amplitudes,and mean frequency change rate of LES and the gastric emptying rate;decreased stomach weight;and improved the LES ***,HXTJD increased the number of ICC-positive cells,and c-kit,Cx43,and SCF expression *** esophageal inflammation,HXTJD significantly decreased the percentage of Th17 cells,and IL-6 and IL-17 concentrations,and increased the percentage of Treg cells and IL-10 ***:HXTJD was found to be efficacious in the rat RE *** may promote esophageal motility and alleviate the inflammatory response by activating the SCF/c-kit/ICC
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