Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress
作者机构：State Key Laboratory of Aridland Crop Science/College of Life Science and Technology Gansu Agricultural University College of Biology and Geographical Sciences Yili Normal University Western Agricultural Research Center Chinese Academy of Agricultural Sciences
出 版 物：《Journal of Integrative Agriculture》 (农业科学学报(英文版))
年 卷 期：2024年第10期
页 面：3370-3386页
核心收录：
基 金：supported by the State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, China (GSCS-2019-10) the National Natural Science Foundation of China (31801414 and 32260478) the Gansu Province Science and Technology Program, China (20JR10RA531) the Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (2022D01E103) the Education Technology Innovation Project of Gansu Province, China (2022QB-076)
摘 要：Activity of bc1 complex kinase(ABC1K) is an atypical protein kinase(aPK) that plays a crucial role in plant mitochondrial and plastid stress responses, but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.). Here, we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.) and found that the Gh ABC1Ks were unevenly distributed across 17 chromosomes. The GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental duplication. The GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress responses. The qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different stresses. Gh ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress treatments. These genes were further functionally characterized by virus-induced gene silencing(VIGS). Compared with the controls, the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA) contents, lower catalase(CAT), peroxidase(POD) and superoxide dismutase(SOD) activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG stress. In addition, the expression levels of six stress marker genes(Gh DREB2A, Gh SOS1, Gh CIPK6, Gh SOS2, Gh WRKY33, and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced lines. The results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG *** findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses.
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