Lovastatin increases nitric oxide synthesis in IL-1β-stimulated smooth muscle cells

作     者：陈红 邢燕 刘如辉 CHEN Hong;XING Yan;LIU Ruhui

作者机构：北京大学人民医院心内科北京100044 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2001年第114卷第11期

页      面：3-7,101页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：HMG  CoA reductase inhibitor · inducible nitric oxide synthase · atherosclerosis 

摘      要：Objective Nitric oxide(NO)production by inducible NO synthase(Inos)may play an important role in the pathogenesis of *** lovastatin has been shown to reduce the progression of atherosclerosis,it is not known whether it regulates NO *** investigated the effects of lovastatin on NO synthesis and the mechanisms by which lovastatin exerts its effects in rat vascular smooth muscle *** Primary cultures of the vascular smooth muscle cells were obtained from the media of the thoracic aorta of Sprague Dawley rats(200 - 250 g).Nitrite levels in the culture medium of rat vascular smooth muscle cells were determined *** Lovastatin(10-5 mol/L)significantly increased interieukin-1β(IL-1β,10 ng/Ml)-induced nitrite accumulation in a time(0- 24 hours)-dependent *** mevalonate and geranylgeranylpyrophosphate completely reversed the stimulatory effects of lovastatin on nitrite ***,inhibition of Rho by C3 exoenzyme mimicked the increase in IL-1β-induced nitrite accumulation induced by lovastatin in the vascular smooth muscle *** These results demonstrate that lovastatin up-regulates NO formation in rat vascular smooth muscle cells stimulated by IL-1β,and the effect may be associated with the inhibition of Rho activity.