Regulating expressions of cyclin D1,pRb,and anti-cancer effects of deguelin on human Burkitt's lymphoma Daudi cells in vitro

作     者：Hong-li L1U Yan CHEN~2 Guo-hui CUI Qiu-ling WU Jing HE Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science arid Technology,Wuhan 430022,China 

作者机构：Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2005年第26卷第7期

页      面：873-880页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(No 30472267) 

主　　题：deguelin Daudi cells cyclin D1 pRb apoptosis 

摘      要：Aim:To investigate anticancer effects and molecular mechanism of deguelin onhuman Burkitt’s lymphoma Daudi cells in vitro and compare the cytotoxicities ofdeguelin on Daudi cells and human peripheral blood monocular cells(PBMC).Methods:The effects of deguelin on the growth of Daudi cells were studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium(MTT)*** dectected through Hoechst 33258 staining and Annexin V/PI *** effect of deguelin on the cell cycle of Daudi cells were studied bya propidium iodide *** expressions of cyclin D 1 and pRb were checkedby Western ***:The proliferation of Daudi cells were decreased indeguelin-treated group with a 24-h IC50value of 51.55 nmol/*** inducedDaudi cells apoptosis was in a time-and dose-dependent manner.G0/G1phaseincreased and S phase decreased in Daudi cells treated with *** deguelin0,5,10,20,and 40 nmol/L treatment for 24 h,G0/G1phase increased from 37.34% to56.56%,whereas S phase decreased from 37.72% to 21.36%.PBMC was lesssensitive to the cytotoxic effect of deguelin than Daudi *** expression ofcyclin D 1 and pRb protein were decreased sharply in Daudi cells treated ***:Deguelin is able to inhibit the proliferation of Daudi cellsby regulating the cell cycle that arrested cells at G0/G1phase and inducing the ***,deguelin selectively induced apoptosis of Daudi cells withlow toxicity in *** antitumor effects of deguelin were related to down-regulating the expression of cyclin D1 and pRb protein.