Frequencies and Characterization of HBV-specific Cytotoxic T Lymphocytes in Self-limited and Chronic Hepatitis B Viral Infection in China

作     者：杨新星 郝友华 刘贽 陈玲 丁红晖 赵西平 陆蒙吉 杨东亮 Xinxing YANG1,Youhua HAO1,Zhi LIU1,Ling CHEN1,Honghui DING1,Xiping ZHAO1,2,Mengji LU3,4,Dongliang YANG1 1Division of Clinical Immunology,2Deparment of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China 3Department of Microbiology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China 4Institute of Virology,Medical School of Duisburg-Essen University,Essen 45122,Germany

作者机构：Division of Clinical ImmunologyTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Deparment of Infectious DiseasesTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of MicrobiologyTongji Medical CollegeHuazhong University of Science and Technology Institute of VirologyMedical School of Duisburg-Essen University 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2009年第29卷第5期

页      面：567-574页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by grants from the National Key Basic Research Program of China (No.20014CB510008,No.2005CB522901) the National Natural Sciences Foundation of China (No.30400412) 

主　　题：hepatitis B virus cytotoxic T lymphocyte HLA-A*0201 

摘      要：Hepatitis B virus （HBV）-specific cytotoxic T lymphocytes （CTLs） are believed to play a major role in viral clearance and disease pathogenesis during HBV infection. To clarify the differences in host immune responses between self-limited and chronic HBV infections, we constructed three HLA-A＊0201/HBV tetramers with immunodominant epitopes of core18-27, polymerase 575-583 and envelope 335-343, and analyzed the HBV-specific CTLs in peripheral blood mononuclear cells （PBMCs） from patients infected with HBV. The frequencies and expansion ability of HBV-specific CD8＋T cells in most self-limited HBV infected individuals were higher than those in chronic HBV-infected patients. HBV-specific CD8＋T cells could be induced by in vitro peptide stimulation from chronic patients with a low level of serum HBV-DNA but not from those with a high level of serum HBV-DNA. In chronic infection, no significant correlation was found either between the frequencies of HBV-specific CD8^＋ T cells and the viral load, or between the frequencies and the levels of alanine transaminase. Our results suggested that the frequencies of HBV-specific CTLs are not the main determinant of immune-mediated protection in chronic HBV infection and immunotherapeutic approaches should be aimed at not only boosting a HBV-specific CD8^＋T response but also improving its function.