The RFA regulatory sequence-binding protein in the promoter of prostate-specific antigen gene

作     者：陈蔚文 张建业 Charles Y F Young 张莲英 陈留存 赵健 

作者机构：Institute of Biochemistry and Molecular Biology School of Medicine Shandong University Jinan 250012 China Department of Urology Research of Mayo Clinic/Foundation MN 55905USA Department of Thoracic Surgery Qilu Hospital of Shandong University Jinan 250012 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2003年第46卷第2期

页      面：184-193页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：prostate-specific antigen, promoter, heterogeneous nuclear ribonucleoprotein A1, A2. 

摘      要：To assure what sequence associated with the androgen regulation, a 15 bp region at the upstream of the ARE of prostate-specific antigen (PSA) promoter, termed RFA, was found in-dispensable for androgen receptor (AR)-mediated transactivation of PSA promoter. In transfection and CAT assays, some nucleotides substitution in RFA could significantly decrease the androgen inducibility for PSA promoter. The in vitro DNA binding assay demonstrated that RFA bound spe-cifically with some non-receptor protein factors in prostate cell nucleus, but the mutant type of RFA lost this ability, so RFA might be a novel accessory cis-element. The RFA-binding proteins were isolated and purified by affinity chromatography using RFA probes. SDS-PAGE and preliminary protein identification showed these proteins possessed sequence high homology with multifunc-tional protein heterogeneous nuclear ribonucleoprotein A1, A2 (hnRNP A1, A2). RFA-binding pro-teins possibly cooperate with AR-mediated transactivation for PSA promoter as coactivator. The study results will facilitate further understanding the mechanism and tissue specificity of PSA pro-moter.